Generator
Part:BBa_K620002
Designed by: Caltech iGEM 2011 Group: iGEM11_Caltech (2011-09-20)
LacI promoter + BisdA
Allows for inducible expression of the protein BisdA, one of a pair of proteins that can degrade bisphenol A (BPA). It is controlled by the lacI promoter, which can be induced by IPTG. It should be used in conjuction with K620003 to degrade BPA.
Usage and Biology
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 216
Illegal AgeI site found at 534 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 407
Illegal BsaI site found at 443
[edit]
Categories
Parameters
None |